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goat anti human sdf 1b polyclonal ab  (R&D Systems)


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    R&D Systems goat anti human sdf 1b polyclonal ab
    FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit <t>SDF-1</t> pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, <t>SDF-1b,</t> IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.
    Goat Anti Human Sdf 1b Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+sdf+1b+polyclonal+ab/Human+CXCL12%2FSDF-1+Antibody/pm11313418-71-10-15
    Average 94 stars, based on 34 article reviews
    goat anti human sdf 1b polyclonal ab - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing."

    Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.166.9.5749

    FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit SDF-1 pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, SDF-1b, IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.
    Figure Legend Snippet: FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit SDF-1 pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, SDF-1b, IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.

    Techniques Used: Recombinant, Expressing, Enzyme-linked Immunosorbent Assay, Chemotaxis Assay, Bioassay, Rnase Protection Assay

    Related Articles

    Recombinant:

    Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.
    Article Snippet: Plates were washed three times with wash buffer.Plates were washed three times with wash buffer.. Next, 100 ml of a 1 mg/ml solution of biotinylated, goat anti-human SDF-1b polyclonal Ab (R&D Systems) was added.. Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.

    Expressing:

    Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.
    Article Snippet: Plates were washed three times with wash buffer.Plates were washed three times with wash buffer.. Next, 100 ml of a 1 mg/ml solution of biotinylated, goat anti-human SDF-1b polyclonal Ab (R&D Systems) was added.. Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.
    Article Snippet: Plates were washed three times with wash buffer.Plates were washed three times with wash buffer.. Next, 100 ml of a 1 mg/ml solution of biotinylated, goat anti-human SDF-1b polyclonal Ab (R&D Systems) was added.. Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.

    Chemotaxis Assay:

    Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.
    Article Snippet: Plates were washed three times with wash buffer.Plates were washed three times with wash buffer.. Next, 100 ml of a 1 mg/ml solution of biotinylated, goat anti-human SDF-1b polyclonal Ab (R&D Systems) was added.. Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.

    Bioassay:

    Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.
    Article Snippet: Plates were washed three times with wash buffer.Plates were washed three times with wash buffer.. Next, 100 ml of a 1 mg/ml solution of biotinylated, goat anti-human SDF-1b polyclonal Ab (R&D Systems) was added.. Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.

    Rnase Protection Assay:

    Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.
    Article Snippet: Plates were washed three times with wash buffer.Plates were washed three times with wash buffer.. Next, 100 ml of a 1 mg/ml solution of biotinylated, goat anti-human SDF-1b polyclonal Ab (R&D Systems) was added.. Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.Plates were incubated for 2 h at 22°C and were washed three times with wash buffer.



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    goat anti human sdf 1b polyclonal ab  (R&D Systems)
    94
    R&D Systems goat anti human sdf 1b polyclonal ab
    FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit <t>SDF-1</t> pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, <t>SDF-1b,</t> IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.
    Goat Anti Human Sdf 1b Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+sdf+1b+polyclonal+ab/Human+CXCL12%2FSDF-1+Antibody/pm11313418-71-10-15
    Average 94 stars, based on 1 article reviews
    goat anti human sdf 1b polyclonal ab - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

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    FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit SDF-1 pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, SDF-1b, IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.

    doi: 10.4049/jimmunol.166.9.5749

    Figure Lengend Snippet: FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit SDF-1 pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, SDF-1b, IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.

    Article Snippet: Next, 100 ml of a 1 mg/ml solution of biotinylated, goat anti-human SDF-1b polyclonal Ab (R&D Systems) was added.

    Techniques: Recombinant, Expressing, Enzyme-linked Immunosorbent Assay, Chemotaxis Assay, Bioassay, Rnase Protection Assay